| Person | Type | Job | Role(s) |
|---|---|---|---|
 Adam Nimoy | Crew | Directing | Director, Writer |
 Kevin Layne | Crew | Camera | Director of Photography, Producer |
 Nicholas Pike | Crew | Sound | Original Music Composer |
David Zappone | Crew | Production | Executive Producer |
Joseph Kornbrodt | Crew | Production | Producer |
Nathan Whitcomb | Crew | Sound | Sound Mixer, Sound Recordist |
Kai De Mello-Folsom | Crew | Production | Production Manager, Sound Recordist, Co-Producer |
Adam Malin | Cast | Self | |
Adam Nimoy | Cast | Actor | Self |
 Avery Brooks | Cast | Actor | Benjamin Sisko (archive footage) |
 Barry Newman | Cast | Actor | Self |
 Bill Prady | Cast | Actor | Himself |
 Brooke Adams | Cast | Actress | Elizabeth Driscoll (archive footage) |
 Catherine Hicks | Cast | Actress | Herself |
 Christopher Lloyd | Cast | Actor | Commander Kruge (archive footage) |
 D.C. Fontana | Cast | Actress | Self |
 Donald Sutherland | Cast | Actor | Matthew Bennell (archive footage) |
 George Takei | Cast | Actor | Himself |
 J.J. Abrams | Cast | Actor | Self |
 James Arness | Cast | Actor | Matt Dillon (archive footage) |
 James Doohan | Cast | Actor | Scotty (archive footage) |
 James Duff | Cast | Actor | Himself |
 Jason Alexander | Cast | Actor | Self |
 Jeanne Bal | Cast | Actress | Nancy Crater (archive footage) |
 Jeffrey Hunter | Cast | Actor | Captain Christopher Pike (archive footage) |
 Jeffrey Katzenberg | Cast | Actor | Self |
 Jill Ireland | Cast | Actress | Leila Kalomi (archive footage) |
 Kaley Cuoco | Cast | Actress | Penny (archive footage) |
 Karl Urban | Cast | Actor | Self |
 Leonard Nimoy | Cast | Actor | Self (archive footage) |
 Liam Neeson | Cast | Actor | Leo Cutter (archive footage) |
 Majel Barrett | Cast | Actress | Number One (archive footage) |
 Mayim Bialik | Cast | Actress | Self |
 Nichelle Nichols | Cast | Actress | Self |
 Nicholas Meyer | Cast | Actor | Himself |
 Peter Duryea | Cast | Actor | Lt. Jose Tyler (archive footage) |
 Simon Pegg | Cast | Actor | Self |
 Ted Danson | Cast | Actor | Jack (archive footage) |
Terry Farrell | Cast | Actress | Jadzia Dax (archive footage) |
 Tom Selleck | Cast | Actor | Peter (archive footage) |
 Walter Koenig | Cast | Actor | Self |
 William Shatner | Cast | Actor | Self |
 William Windom | Cast | Actor | Commodore Decker (archive footage) |
 Zachary Quinto | Cast | Actor | Self |
 Zoe Saldaña | Cast | Actress | Self |
 Chris Pine | Cast | Actor | Self |
 Jim Parsons | Cast | Actor | Self |
 Neil deGrasse Tyson | Cast | Actor | Self |
Julie Nimoy | Cast | Self | |
Bobak Ferdowsi | Cast | Actor | Self |
Scott Mantz | Cast | Actor | Self |
 Diana Ewing | Cast | Actress | Droxine (archive footage) |
| Person | Type | Job | Role(s) |
